cdnas  (OriGene)

Journal: International Journal of Molecular Sciences

Article Title: Expression of DNA Methyltransferase 3B Isoforms Is Associated with DNA Satellite 2 Hypomethylation and Clinical Prognosis in Advanced High-Grade Serous Ovarian Carcinoma

doi: 10.3390/ijms232112759

Figure Lengend Snippet: DNMT3B3 overexpression validated in advanced stages of high-grade serous ovarian carcinoma (HGSOC). DNMT3B3 isoform overexpression in two arrays of cDNA rapid cDNA panels: II and III, of ovarian carcinoma (TissueScan Ovarian Cancer cDNA arrays II and III) (OriGene Technologies, Rockville MD) (cat. no. HORT102, and 103) (n = 82). Non-advanced HGSOC (n = 23: mucinous, low-grade serous ovarian carcinoma, endometrioid, carcinoma clear cells, and stages I–II of HGSOC) versus advanced HGSOC (n = 59: stages III–IV of HGSOC). Assays were normalized with GAPDH expression in each case and ten controls of the normal ovary. Mann–Whitney U test was used.

Article Snippet: Testing was carried out in two arrays of rapid cDNA panels: II and III, of ovarian carcinoma (TissueScan Ovarian Cancer cDNA arrays II and III) (OriGene Technologies, Rockville, MD, USA) (cat. no. HORT102 and 103) (n = 82).

Techniques: Over Expression, Expressing, MANN-WHITNEY

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Selective targeting of metastatic ovarian cancer using an engineered anthrax prodrug activated by membrane-anchored serine proteases.

doi: 10.1073/pnas.2201423119

Figure Lengend Snippet: Fig. 6. Potent antitumor cytotoxicity of PAS:LF toxin in patient-derived primary human tumors. (A) Real-time qPCR analysis showing increased hepsin, testisin, and matriptase mRNA expression in patients with OvCa of various histological type and stage of tumorigenic development (Human Ovarian Cancer TissueScan Array; Origene) compared with normal ovary tissue. Data are representedin box plots showing the median, lower and upper quartiles (horizontal lines) of rela- tive expression +SEM compared to normal ovary tissue normalized to β-actin (*P < 0.05, ***P < 0.005, ****P < 0.001). (B) Tumor cells recovered from ascites fluids from five patients were collected by centrifugation, contaminating red blood cells were removed by hypotonic lysis, and cytospins were assessed for tumor purity by Kwik-Diff stain and expression of the OvCa markers CA125 and PAX8. Images are 40X original magnification. Insets are close-up images of indi- vidual cells or clusters. The tumor cell percentage of total cells in ascites fluid was quantified using ImageJ. The chemotherapy (Chemo) treatment status, histo- logical subtype, tumor stage, and primary tumor origin were determined by independent staff pathologists as part of the clinical diagnosis. HGSOC, high-grade serous ovarian carcinoma; LGSOC, low-grade serous ovarian carcinoma. (C) (i) The recovered human ascites tumor cells were seeded on agarose-coated plates for 24 h, where they formed organotypic multicellular spheroids. Spheroids that were treated twice with PAS:LF (Patients #1 and #2: 2 μg/mL, Patients #3 to #5: 6 μg/mL) for a total of 120 h. Spheroid viability relative to untreated controls was quantified via PrestoBlue or ImageJ quantification of decrease in size and is shown as an average of the five patient samples ± SEM performed in three to six replicates (****P < 0.001; ns, not significant). (ii) Representative images of spheroids from Patient #4 and Patient #5 after 120 h of treatment are shown. Scale bars = 650μm.

Article Snippet: Potent antitumor cytotoxicity of PAS:LF toxin in patient-derived primary human tumors. (A) Real-time qPCR analysis showing increased hepsin, testisin, and matriptase mRNA expression in patients with OvCa of various histological type and stage of tumorigenic development (Human Ovarian Cancer TissueScan Array; Origene) compared with normal ovary tissue.

Techniques: Derivative Assay, Expressing, Centrifugation, Lysis, Staining, Biomarker Discovery